Structural analysis and genetic variation of the 16S-23S rDNA internal spacer region from Micrococcus luteus strains

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Abstract

Aims: To clone and sequence the 16S-23S ribosomal DNA (rDNA) internal spacer region (ISR) from Micrococcus luteus. Methods and Results: The primer pair for 16S-23S rDNA ISR amplified a fragment of about 850 bp in length for two strains, JCM3347 and JCM3348 and a fragment of about 790 bp for a strain, ATCC9341. After sequencing the ISRs were identified by the comparison of the ISRs and the flanking regions of ISR. Conclusions: Although the sequence difference of the ISR occurred at only one position between the two JCM strains, the highly variable length (440 and 370 bp) and sequence similarity (about 40%) were demonstrated between the ISRs of the two JCM strains and a ATCC strain. Significance and Impact of the Study: A CCTCCT sequence was first detected at the 3′-end of the 16S rDNA of the three strains. Moreover, highly similar sequence to the 21-bp region containing a putative rRNA processing site was observed in the ISR of the three strains. Interestingly, no intercistronic tRNAs were demonstrated in the ISRs from the three strains.

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Haga, S., Hirano, Y., Murayama, O., Millar, B. C., Moore, J. E., & Matsuda, M. (2003). Structural analysis and genetic variation of the 16S-23S rDNA internal spacer region from Micrococcus luteus strains. Letters in Applied Microbiology, 37(4), 314–317. https://doi.org/10.1046/j.1472-765X.2003.01399.x

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