Effects of temperature and glyphosate on fatty acid composition, antioxidant capacity, and lipid peroxidation in the gastropod lymneae sp.

Abstract

Little is known about the potential effects of glyphosate on freshwater gastropods and possible interactions between glyphosate and other stressors. A two-way factorial experiment was conducted to investigate the effects of temperature (20◦ C/25◦ C) and glyphosate (0 µg/L/200 µg/L) on Lymnaea sp. After 21 days, antioxidant capacity (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST)), malondialdehyde content (MDA), and fatty acid (FA) composition of Lymnaea sp. tissue were measured. Temperature had an effect on SOD activity and GPx activity. In contrast, an increase in GST activity was observed in glyphosateexposed snails, highlighting the role of GST in the glyphosate detoxification process. Differences in temperature and glyphosate did not affect lipid peroxidation (MDA); however, we observed a trend suggesting the presence of higher MDA content in glyphosate-exposed snails at 20◦ C. The FA groups were generally not strongly affected by the treatments, except for omega−9 (n-9) that was markedly lower at the higher temperature. Changes were also observed in individual FA as a response to glyphosate and/or temperature. For example, a significant decrease in 18:1n9 was observed at 25◦ C. Our results showed that antioxidant capacity and FA profiles were mainly affected by temperature, while glyphosate seemed to have a lesser impact.