Characterization of Aeromonas sobria Isolated from Fish Rohu (Labeo rohita) Collected from Polluted Pond

Abstract

One of the major threats to fish aquaculture sector is the infection by Aeromonas Spp. The current study assesses the phenotypic characteristics and biochemical characterization of the A. sobria strains from the fish cases with septicaemia in order to understand the frequency and occurrence of this infection in the state of Jammu and Kashmir. Clinically the infected fish Rohu (Labeo rohita), one of the Indian Major carps (IMC), was observed for symptoms like loss of escape reflex and skin darkness associated with skin haemorrhages. We isolated 30 colonies of A. sobria strain from 10 cultured Labeo rohita collected from a controlled fish pond in District Poonch of the state. The pond was affected by mismanagement practices, elevated pollution levels and anthropogenic activities. Microscopic examination revealed that the strain was rod-shaped and gram negative. The revealed percent probability identification of A. sobria from the biochemical characterization in Vitek system was 93% with GN card. This study could give us clues for understanding A. sobria harbouring in fish species and shall help in better understanding of the threat prevalent to the fish species of the region by this infection.