Allocation to clonal and sexual reproduction and its plasticity in Vallisneria spinulosa along a water-depth gradient

Abstract

Variations in water level profoundly affect functional stability of freshwater ecosystems, as well as macrophyte growth and reproduction. Although the trade-off between allocation to clonal and sexual reproduction in clonal plants can be influenced by a variety of environmental factors, whether variations of reproductive allocation (RA) in response to different environments are driven only by a sizedependent effect (apparent plasticity) or whether RA can also change independently of plant size (true plasticity) is uncertain. We conducted an experiment in nine outdoor mesocosms (6400 L) to investigate the response of clonal and sexual reproduction and vegetative growth of a perennial submerged macrophyte Vallisneria spinulosa at water depths of 50, 100, or 150 cm. We evaluated size-dependent and sizeindependent effects of water depth on sexual and clonal RA. Deep water reduced vegetative size and sexual output (mass of fruits produced), but increased tuber production of V. spinulosa. There was an apparent trade-off between reproductive modes in terms of biomass investment; plants in deep water allocated more resources into clonal propagation and reduced investment in sexual reproduction compared to plants in shallow or intermediate water. Slopes of allometric relationships (sexual vs. vegetative biomass and clonal vs. vegetative biomass) were significantly affected by water depth. Shifts in sexual RA in response to varying water depths were largely size-dependent, but there were also size-independent effects. In contrast, size-independent effects were more important than effects of size changes in determining clonal RA. We concluded that V. spinulosa adapted to a water-depth gradient by plastic trade-offs between clonal propagation and sexual reproduction. Furthermore, a size-independent effect on RA suggests a flexible reproductive strategy that could be critical for plant performance in changing aquatic environments.