Using cutaneous leishmaniasis of mice, the existence of so-called T helper (Th) cells type 1 and type 2 had been identified more than 20 years ago. Nowadays, it is well accepted that additional T cell populations as well as B cell-mediated immunity is required for immunity against Leishmania major . Finally, using inbred mouse strains, the relevance of genetical factors that influence anti-pathogen immunity as well as elements of the skin-immune system have been identified. This protocol describes a model for murine experimental leishmaniasis that tries to mimic natural parasite transmission by several means: (1) utilization of only infectious-stage parasites that are found in sandfly saliva, (2) intradermal inoculation, and (3) infection with only 1,000 parasites similar to the numbers inoculated by an infected sandfly. © Springer Science+Business Media, LLC 2013.
CITATION STYLE
Fischer, M. R., John, D., Kautz-Neu, K., Schermann, A. I., Schwonberg, K., & Von Stebut, E. (2013). Animal model for cutaneous leishmaniasis. Methods in Molecular Biology, 961, 389–402. https://doi.org/10.1007/978-1-62703-227-8_26
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