Investigating on the Effective Wound Healing Therapy Utilizing Laser Optical System

Abstract

Recent advances in wound healing treatment and management using Low- level Laser Therapy (LLLT) has been emphasized in many types of research for wound healing stimulation. Many studies have indicated the effects of this method; and thus, there is a concern that which wavelength and dosages would take more benefits, in term of wound closure and collagen synthesis. This research is conducted to re-emphasize and improve the efficiency of this therapy in chronic wounds and standard rat model to study and analyze the best conditions and protocol for these kinds of wound healing treatment. By utilizing red laser beams from an optical system to treat excisions on mice’s dorsal regions, we aim to determine the irradiation parameters (i.e., wavelength, light intensity, time irradiation) that provide the optimal conditions to promote the healing process. Two round excision wounds are created on the back of each mouse: one for a control group and one for treated group. The treated group wound were irradiated with the red (635 nm wavelength), following these intensities: 2; 3; 5 J/cm2. After every one week, the wound skins were taken and stained for results comparison. The experimental results showed that the wound closures were significantly different between treated and control group. At day 5th, 10th the follow-up tests indicated that the laser group had smaller wound areas compared to control group (non-diabetes and untreated). The improvement of impairment (>20%) and the faster time to wound closure indicated that this phototherapy could be used to heal chronic wounds in diabetic subjects. The healing progress analysis shows that the positive result and promising application of this therapy in future aid of wound control and healing.