Zebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafish, using a coffee plunger and magnetic stirrer. To demonstrate the use of this method, we conducted a pilot experiment to show that this simple system provides repeatable estimates of maximal swim performance (intra-class correlation [ICC] = 0.34-0.41) and observe that exercise training of zebrafish on this system significantly increases their maximum swimming speed. We propose this high-throughput and reproducible system as an alternative to traditional linear chamber systems for exercising zebrafish and similarly sized fishes.
CITATION STYLE
Usui, T., Noble, D. W. A., O’Dea, R. E., Fangmeier, M. L., Lagisz, M., Hesselson, D., & Nakagawa, S. (2018). The French press: A repeatable and high-throughput approach to exercising zebrafish (Danio rerio). PeerJ, 2018(1). https://doi.org/10.7717/peerj.4292
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