Performing electrophysiological recordings from human neurons that have been differentiated in vitro, as compared to primary cultures, raises many challenges. However, patch-clamp recording from neurons derived from stem cells provides an abundance of valuable information, reliably and fast. Here, we describe a protocol that is used successfully in our lab for recording from both control and Fragile X neurons, derived in vitro from human embryonic stem cells.
CITATION STYLE
Telias, M., & Segal, M. (2019). Patch-clamp recordings from human embryonic stem cells-derived fragile X neurons. In Methods in Molecular Biology (Vol. 1942, pp. 131–139). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9080-1_11
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