Development of Thelohanellus hovorkai and Thelohanellus nikolskii (myxosporea: myxozoa) in oligochaete alternate hosts

Abstract

The development of Thelohanellus hovorkai, a myxosporean parasite of the connective tissues, and Thelohanellus nikolskii, a fin and scale parasite of common carp (Cyprinus carpio) was studied in experimentally infected oligochaetes Branchiura sowerbyi and Tubifex tubifex, respectively. After infection with mature spores of T. hovorkai, the development of actinosporean stages was first observed light microscopically in the gut of Branchiura sowerbyi 93 days after infection. Free actinospores of T. hovorkai were found in the lumen of the oligochaete's gut 101 days after infection. They were floating in water and showed a typical aurantiactinomyxon form. At 18-22°C, aurantiactinomyxon spores of T. hovorkai emerged from the worms 104 days after infection. The development of T. nikolskii was examined in Tubifex tubifex, from which aurantiactinomyxon spores were released 60 days after infection at 22-24°C. The diameter of spore body was 18.6 μm and the lenght of caudal processes 29 μm for T. hovorkai, while 21.1 μm and 13.4 μm for T. nikolskii, respectively. The prevalence of aurantiactinomyxon infection in B. sowerbyi for T. hovorkai proved to be 16.7%, while in T. tubifex for T. nikolskii it was 12.5%.