Comparative HPTLC analysis of bioactive marker barbaloin from in vitro and naturally grown Aloe vera

Abstract

Aloe vera (L.) Burm. f., Xanthorrhoeaceae, a succulent, produces barbaloin, a bioactive compounds usedin various pharmaceutical products. Extracts prepared from the leaves have been widely used as bitter-ing agents, taste modifiers and also as cathartic agent against severe constipation. Barbaloin is reportedfor its anti-inflammatory, anticancer, antiviral and anticancer activities and these properties are mostlymediated by its antioxidative capacity. Presently, a study has been conducted on the comparative HighPerformance Thin Layer Chromatography analysis of barbaloin from the dried leaf skin powder of in vivoand in vitro grown A. vera. Shoot tips of A. vera were cultured in Murashige and Skoog media supplementedwith different combination of 6-benzylaminopurine and 1-naphthaleneacetic acid. [Best multiplicationresponse was noted in benzylaminopurine (2.0 mg/l) + 1-naphthaleneacetic acid (0.1 mg/l) supplementedMurashige and Skoog media]. The quantitative determination of barbaloin was performed on silica gel 60F254 HPTLC plates as stationary phase. The linear ascending development was carried out in a twin troughglass chamber saturated with a mobile phase consisting of ethyl acetate: Methanol: Water (100:16.5:13.5)at room temperature (22 ± 2◦C). CAMAG Thin Layer Chromatography scanner-3 equipped with CATS software (version: 1.4.4.6337) was used for spectrodensitometric scanning and analysis in the ultravioletregion at λ = 366 nm. The method was validated for linearity, precision and accuracy. Correlation coefficient, limit of detection, limit of quantification as well as recovery values were found to be satisfactory. Out of the five populations studied, the leaf skin of A. vera collected from Jodhpur (Rajasthan, India) andraised in vitro was found to contain higher amount of barbaloin (2.78%) when compared to its naturallygrowing counterparts (2.46%) and other plant populations.