The immunological effects of continuous veno-venous haemodiafiltration in critically ill patients

Abstract

Background: Haemodynamic instability is common in septic patients with acute renal failure. Continuous veno-venous haemodiafiltration (CWHD) is therefore used as an alternative to conventional haemodialysis. Haemodialysis is associated with an activation of the immune system. The aim of the present study was to test the hypothesis that initiation of CVVHD influences the immune system with release of proinflammatory cytokines followed by a decrease in granulocyte activation, as assessed by the expression of adhesion molecules. Results: Fifteen patients were included. Mean Acute Physiology and Chronic Health Evaluation-2 score before CWHD was 19 (range 8-27). Mean duration of CVVHD treatment was 9 days (1-21 days). Tumour necrosis factor-α and interleukin-8 were detectable in plasma in all patients, whereas interleukin-10 was detectable only in a few patients. Proinflammatory and anti-inflammatory cytokines were detected in the ultrafiltrate. Large intraindividual and interindividual variations were demonstrated for all of the immunological parameters studied. Conclusion: The hypothesis that CVVHD induces the release of proinflammatory cytokines followed by a decrease in granulocyte activation was not confirmed in the present study. The heterogeneous group of patients studied, with different underlying diseases and various durations of illness before the start of CWHD, might have contributed to the difficulty in demonstrating the proposed immunological effect of CWHD.