Method for Human Eccrine Sweat Gland Isolation from the Scalp by Means of the Micropunch Technique and Vital Dyes

Abstract

The isolation of eccrine sweat glands from human skin has always been a difficult task. The human scalp contains thousands of eccrine glands. Recently, the close anatomic relationship of the eccrine gland with the scalp hair follicle has been described. Taking advantage of this anatomic relationship as well as of the availability of follicular units (FUs) obtained in hair transplant procedures, we describe here a simple and efficient method to isolate eccrine sweat glands from the human scalp. This method is identical to the micropunch hair graft harvesting method known as follicular unit excision (FUE), used in modern hair transplantation. Once the FU has been extracted, it needs to be stained with methylene blue or neutral red in order to make the sweat gland visible for stereoscopic microdissection. Only the secretory (coiled) portion of the sweat gland can be obtained with this method. The efficiency of this isolation method should encourage further research into human eccrine sweat glands and opens possibilities for new translational applications.