Processing protocols for high quality glass-based microarrays: applications in DNA, peptide, antibody, and carbohydrate microarraying.

Abstract

Based on the selection of a suitable surface chemistry and bearing the option for optimization using a defined workflow, standard experimental protocols for the processing of microarrays can be used as starting points for a successful experiment. In Chapters 2 and 3, general quality considerations and the selection of surface chemistry have been discussed. A workflow for the selection of slide surface architectures and the optimization of microarray processing parameters also has been described. In the present article, processing parameters for DNA, peptide, antibody, and carbohydrate microarrays are outlined that serve as a first recommended step in the iterative establishment process. For a number of popular applications of microarray technology the outlined protocols can be applied to directly generate high-quality results.