Tissue decellularization allows isolation of tissue extracellular matrix components, enabling bioengineering of native tissue microenvironments with minimal antigenic components. Here we describe a method to harvest decellularized cartilage tissue from donor trachea using a series of chemical and enzymatic washes and incorporating the extracellular matrix in gelatin methacrylate hydrogels. This decellularized cartilage tissue is easily incorporated into a variety of hydrogels to create a cartilage tissue scaffold.
CITATION STYLE
Galliger, Z., & Panoskaltsis-Mortari, A. (2018). Tracheal cartilage isolation and decellularization. In Methods in Molecular Biology (Vol. 1577, pp. 155–160). Humana Press Inc. https://doi.org/10.1007/7651_2017_52
Mendeley helps you to discover research relevant for your work.