PGD for sex determination and chromosome rearrangements: Fish and emerging technologies

Abstract

Fluorescence in situ hybridisation (FISH) on the fixed nuclei of biopsied blastomeres from day 3 cleavage-stage embryos using target-specific DNA probes has been for over a decade the technique of choice for detecting chromosome imbalance in embryos from couples referred for PGD because of a chromosome rearrangement. It has also been used to select female embryos in families with the rare X-linked diseases for which a mutation-specific test was not available or practical.