The introduction of genetically modi fied plants into natural habitats represents a valuable means to determine organismic level functions of a gene and its effects on a plant's interaction with other organisms. Nicotiana attenuata a wild tobacco species native of the southwestern USA that grows in the immediate post fire environment, is one of the important host plants for herbivore populations recolonizing recently burned habitats in the Great Basin Desert. Here, we provide detailed guidelines for the analysis, under field conditions, of jasmonate-dependent defense and its impact on the plant's native herbivore community. The procedures are based on the field release of transgenic lines silenced for jasmonate biogenesis, metabolism, or perception to conduct association studies between defense trait expression (secondary metabolite and trypsin proteinase inhibitor accumulation) and insect infestations. Additionally, because some insects have evolved mechanisms to "eavesdrop" on jasmonate signaling when selecting their host plants, we describe how leafhoppers of the species Empoasca which selectively colonize jasmonate-de ficient plants, can be used as "bloodhounds" for identifying natural variations in jasmonate signaling among natural N. attenuata populations. © Springer Science+Business Media, LLC 2013.
CITATION STYLE
Gaquerel, E., Stitz, M., Kallenbach, M., & Baldwin, I. T. (2013). Jasmonate signaling in the field, part II: Insect-guided characterization of genetic variations in jasmonate-dependent defenses of transgenic and natural Nicotiana attenuata populations. Methods in Molecular Biology, 1011, 97–109. https://doi.org/10.1007/978-1-62703-414-2_8
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