Micropropagation of Salvia brachyodon through nodal explants

Abstract

A protocol for in vitro propagation of Balkan endemic plant Salvia brachyodon Vandas from nodal segments was developed. 6-benzylaminopurine was more effective in axillary buds promotion when compared to thidiazuron. The rooting of regenerated shoots was induced by transferring them to the media supplemented with auxins. All tested auxins (indole-3-acetic acid, indole-3-butyric acid, and α-naphthaleneacetic acid) stimulated the rooting of S. brachyodon shoots. The acclimatization of in vitro rooted shoots was successful. © 2006 Institute of Experimental Botany, Academy of Sciences of the Czech Republic.