Global characterization of protein complexes by biochemical purification-mass spectrometry (BP/MS)

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Abstract

A proteomic platform for global analysis of protein complexes and protein-protein interactions (PPIs) is described. Briefly, after comprehensive physiochemical separation of soluble protein extracts using non-denaturing ion exchange chromatography (IEX), each fraction is subjected to quantitative tandem mass spectrometry analysis.

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Pourhaghighi, R., & Emili, A. (2018). Global characterization of protein complexes by biochemical purification-mass spectrometry (BP/MS). In Methods in Molecular Biology (Vol. 1764, pp. 185–191). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7759-8_12

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