Altered active but not passive properties of mesenteric resistance arteries from the vitamin E-deprived rat

Abstract

1. We tested the hypothesis that lowering antioxidant protection through dietary vitamin E deprivation would alter active and passive mechanical properties in resistance arteries of the rat. Specifically, we hypothesized that vascular tone in isolated mesenteric arteries of the vitamin E-deprived rats would be altered due to impaired endothelial influences of nitric oxide and/or prostaglandins. 2. Lumen diameter and wall thickness were measured in pressurized arteries (≃ 250 μm diameter) from control (n = 9) and vitamin E deprived (n = 9) Sprague-Dawley female rats by use of a dimension analysing system. 3. Treatment with a cyclo-oxygenase inhibitor (meclofenamate) did not affect the basal vascular tone in either group. Treatment with a nitric oxide synthase inhibitor (N(G)-methyl-L-arginine) caused a significant increase in basal tone only in the vitamin E-deprived rats (% tone: 6.2 ± 1.1 vs 1.2 ± 0.3%, P < 0.05). When tone was induced to 25% of the initial diameter with phenylephrine, treatment with the nitric oxide synthase inhibitor resulted in a greater potentiated lone in the vitamin E-deprived rats compared to the controls (26.5 ± 2.7 vs 16.4 ± 3.4%; P < 0.05); suggesting a greater nitric oxide affect in the vessels from the vitamin E-deprived rats. Meclofenamate treatment in the induced tone arteries significantly relaxed (-17.4 ± 4.0%; P < 0.05) only the arteries from the vitamin E-deprived rats, indicating that a vasoconstrictor was modifying tone. The passive characteristics of distensibility and stress-strain relationship were not different between the two groups of rats. 4. In summary, vitamin E deprivation in the rat enhanced the modulation of vascular tone by both the nitric oxide and cyclo-oxygenase pathways but did not alter passive characteristics of mesenteric arteries.