Flow cytometry is a powerful tool for rapid evaluation of multiple functional properties of large numbers of platelets in whole blood. In the following chapter, we provide a number of flow cytometry-based protocols broadly aimed at (1) assessment of constitutively expressed platelet membrane receptors to diagnose inherited platelet bleeding disorders and (2) investigation of basal and agonist-induced platelet functional responses including generation of platelet-leukocyte aggregates, alpha and dense granule release, calcium flux, and phosphatidylserine exposure.
CITATION STYLE
Pasalic, L., Pennings, G. J., Connor, D., Campbell, H., Kritharides, L., & Chen, V. M. (2017). Flow cytometry protocols for assessment of platelet function in whole blood. In Methods in Molecular Biology (Vol. 1646, pp. 369–389). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7196-1_28
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