Comparing fluorescence-based cell-free assays for the assessment of antioxidative capacity of high-density lipoproteins

9Citations
Citations of this article
11Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Background: Population studies have shown an inverse association between high-density lipoprotein (HDL) cholesterol levels and risk of coronary heart disease (CHD). HDL has different functions, including the ability to protect biological molecules from oxidation. Our aim was to evaluate the performance of two fluorescence-based assays in assessing the antioxidative capacity of HDL. Methods: We compared the antioxidative capacity of HDL with the phospholipid 2',7'-dichlorodihydrofluorescein (DCF) assay and the dihydrorhodamine 123 (DHR) assay in controls and in subjects at increased risk of CHD, including subjects with established CHD, and subjects with elevated plasma triglycerides (TG), serum amyloid A (SAA), or myeloperoxidase (MPO) levels. Results: The antioxidative capacity of HDL, as measured by the DCF assay, was significantly lower in both CHD and high-TG patients than in controls (p < 0.001 and p = 0.004, respectively). Interestingly, the mean antioxidative capacity of HDL in high-SAA subjects was significantly higher (p < 0.03), while in high-MPO subjects was similar to controls. When the DHR assay was used we did not find differences in HDL's antioxidative capacity between CHD patients and controls but we found higher antioxidative capacity in high-SAA subjects compared to controls. Conclusions: Only the DCF assay could detect significant differences in the antioxidative capacity of HDL between controls and CHD subjects. Practical use of both assays for the assessment of antioxidative capacity of HDL is limited by the large overlap in values among groups. The antioxidative activity of HDL in patients who have elevated SAA levels needs to be reassessed.

Figures

  • Table 1 Plasma lipid levels and antioxidative capacity of HDL as measured by the DCF assay in apoBd plasma and serum samples in control subjects
  • Table 2 Plasma or serum lipid profiles in control subjects and patients whose HDL antioxidative activities were measured in apoBd plasma or serum, respectively, with the DCF assay
  • Fig. 1 Distribution and median values of the antioxidant capacity of HDL as assessed by the DCF assay in apoBd plasma samples of apparently healthy subjects (Controls), patients with cardiovascular disease (CVD patients), patients with high triglyceride levels (High TG patients), and patients with high serum amyloid A levels (High SAA patients). Differences between groups were tested by Mann-Whitney U test
  • Table 3 Plasma lipid profiles of control subjects and patients whose HDL antioxidative capacity was measured in ultracentrifuged HDL (UCHDL) and apoBd plasma samples using the DHR assay
  • Fig. 2 Distribution and median values of the oxidation rate of DHR (ORD) of ultracentrifuged HDL fractions (UCHDL) from apparently healthy subjects (Controls) and patients with the cardiovascular disease (CVD patients), and of apoB-deleted (apoBd) plasma from Controls, CVD patients, and patients with high serum amyloid A protein levels (High SAA patients). Differences between groups were tested by Mann-Whitney U test
  • Fig. 3 Correlation between the oxidation rate of DHR (ORD) of ultracentrifuged HDL fraction (UCHDL) and of apoB-depleted (apoBd) plasma in apparently healthy subjects and patients with cardiovascular disease

References Powered by Scopus

Cited by Powered by Scopus

63Citations
49Readers

This article is free to access.

59Citations
48Readers
Get full text
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Tsunoda, F., Lamon-Fava, S., Horvath, K. V., Schaefer, E. J., & Asztalos, B. F. (2016). Comparing fluorescence-based cell-free assays for the assessment of antioxidative capacity of high-density lipoproteins. Lipids in Health and Disease, 15(1), 1–8. https://doi.org/10.1186/s12944-016-0336-y

Readers over time

‘16‘17‘18‘20‘21‘24‘2500.751.52.253

Readers' Seniority

Tooltip

Professor / Associate Prof. 2

33%

PhD / Post grad / Masters / Doc 2

33%

Lecturer / Post doc 1

17%

Researcher 1

17%

Readers' Discipline

Tooltip

Medicine and Dentistry 4

50%

Biochemistry, Genetics and Molecular Bi... 2

25%

Computer Science 1

13%

Psychology 1

13%

Save time finding and organizing research with Mendeley

Sign up for free
0