The BCR-ABL1 fusion gene, the causative lesion of chronic myeloid leukemia (CML) in >95 % of newly presenting patients, offers both a therapeutic and diagnostic target. Reverse-transcription quantitative polymerase chain reaction technology (RT-qPCR), utilizing primer–probe combinations directed to exons flanking the breakpoint junctional region, offers very high levels of both specificity and sensitivity, in a scalable, robust, and cost-effective assay.
CITATION STYLE
Foskett, P., Gerrard, G., & Foroni, L. (2014). Real-time quantification assay to monitor BCR-ABL1 transcripts in chronic myeloid leukemia. Methods in Molecular Biology, 1160, 115–124. https://doi.org/10.1007/978-1-4939-0733-5_11
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