The Abelson murine leukaemia virus protein (P120) can become phosphorylated in vitro by [γ-32P]ATP. The protein has been purified from cell membranes to the point that in specific conditions virtually all of the incorporated 32P is in P120. The reaction is stimulated by Mn 2+ and Mg2+ but not Ca2+ and is very rapid even at 0 °C. The phosphate is linked to P120 at tyrosine, a linkage not previously reported for a phosphorylation reaction. Phosphorylation may be involved in the transforming activity of viruses that cause leukaemia as well as sarcomas. © 1980 Nature Publishing Group.
CITATION STYLE
Witte, O. N., Dasgupta, A., & Baltimore, D. (1980). Abelson murine leukaemia virus protein is phosphorylated in vitro to form phosphotyrosine. Nature, 283(5750), 826–831. https://doi.org/10.1038/283826a0
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