Identification of a new heparin‐binding protein localized within chick basement membranes

Abstract

A protein with a molecular mass of 19 kDa has been purified to homogeneity from 11‐day‐old chick embryos using a procedure involving chromatography on heparin–Sepharose, immunoaffinity resin and C4 reversed‐phase. Indirect immunofluorescence studies, using polyclonal and monoclonal antibodies raised against this protein, indicate that it is essentially localized within the basement membranes in early embryonic tissues. After the 18th day of embryonic life and in post‐hatched chicken, this protein could only be detected in some eye basement membranes. It appears to be bound to heparan sulfate chains of the proteoglycan present in these structures. Thus, the protein exhibits similar properties to those previously described for fibroblast growth factors (FGF). such as heparin affinity, molecular mass and localization in the basement membranes. In contrast, this protein is present in much larger amounts than FGFs, at least in 11‐day‐old embryos. Furthermore, the first 17 amino acid residues of the N‐terminal sequence show that it does not strictly correspond to any previously described protein. Copyright © 1989, Wiley Blackwell. All rights reserved