We describe, and compare, two methods which enable the assembly of proteins from peptide thioester fragments prepared by Fmoc chemistry mediated solid phase synthesis. The first, which utilizes iso-thiouronium salts, allows formation of thiophenyl esters directly from partially protected peptides, either in solution or on resin. The second uses 'sulfonamide' based safety-catch resins. Data on yields, generality and potential for side-reactions are provided. © 2001 Kluwer Academic Publishers.
CITATION STYLE
Biancalana, S., Hudson, D., Songster, M. F., & Thompson, S. A. (2000). Fmoc chemistry compatible thio-ligation assembly of proteins. International Journal of Peptide Research and Therapeutics, 7(5), 291–297.
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