Enthalpy changes during photosynthetic water oxidation tracked by time-resolved calorimetry using a photothermal beam deflection technique

Abstract

The energetics of the individual reaction steps in the catalytic cycle of photosynthetic water oxidation at the Mn4Ca complex of photosystem II (PSII) are of prime interest. We studied the electron transfer reactions in oxygen-evolving PSII membrane particles from spinach by a photothermal beam deflection technique, allowing for time-resolved calorimetry in the micro- to millisecond domain. For an ideal quantum yield of 100%, the enthalpy change, δH, coupled to the formation of the radical pair YZ+QA-(where Y4 is Tyr-161 of the D1 subunit of PSII) is estimated as -820 ± 250 meV. For a lower quantum yield of 70%, the enthalpy change is estimated to be -400 ±250 meV. The observed nonthermal signal possibly is due to a contraction of the PSII protein volume (apparent δ V of about -13 A°3). For the first time, the enthalpy change of the O2-evolving transition of the S-state cycle was monitored directly. Surprisingly, the reaction is only slightly exergonic. A value of δH(S3⇒S0)of-210meV is estimated, but also an enthalpy change of zero is within the error range. A prominent nonthermal photothermal beam deflection signal (apparent A δof about +42 A°3) may reflect O2 and proton release from the manganese complex, but also reorganization of the protein matrix. © 2008 by the Biophysical Society.