ChIP-seq is the current method of choice for genome-wide protein location analysis. Here, we present a native (non-cross-linked) ChIP procedure suitable for histone proteins, coupled with an efficient library preparation technique for subsequent next-generation sequencing. The method enables ChIP-seq starting with 50,000 or more cells.
CITATION STYLE
Ribarska, T., & Gilfillan, G. D. (2018). Native chromatin immunoprecipitation-sequencing (ChIP-Seq) from low cell numbers. In Methods in Molecular Biology (Vol. 1689, pp. 157–166). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7380-4_14
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