Agrobacterium-mediated transformation of barley is a valuable tool for determining gene function by over-expression of a gene of interest or by RNAi-based gene silencing. The method is based on the inoculation of immature embryos with Agrobacterium and uses a hygromycin resistance gene to allow selection of transgenic plants. The protocol described leads to average transformation efficiencies of 25 % meaning that large numbers of fertile transgenic plants can be produced. © 2014 Springer Science+Business Media, New York.
CITATION STYLE
Harwood, W. A. (2014). A protocol for high-throughput agrobacterium-mediated barley transformation. Methods in Molecular Biology, 1099, 251–260. https://doi.org/10.1007/978-1-62703-715-0_20
Mendeley helps you to discover research relevant for your work.