Immunofluorescence or IF is a technique allowing the visualization of a specific protein or antigen in cells or tissues by binding a specific antibody chemically conjugated with a fluorescence dye. Immunofluorescent staining is widely used in life science research, particularly for neuroscience. Here, we describe the immunofluorescent staining of whole-mount neonatal mouse diaphragms to study the morphological patterns of the neuromuscular junction (NMJ) by using of presynaptic neuronal marker-neurofilament (NF) and synaptophysin antibodies; postsynaptic acetylcholine receptors (AChRs) were labeled with Alexa Fluor 594-conjugated α-bungarotoxin (α-BTX). Immunofluorescence- stained diaphragms were examined under a confocal microscope. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Wu, H., & Mei, L. (2013). Morphological analysis of neuromuscular junctions by immunofluorescent staining of whole-mount mouse diaphragms. Methods in Molecular Biology, 1018, 277–285. https://doi.org/10.1007/978-1-62703-444-9_26
Mendeley helps you to discover research relevant for your work.