The Yeast Two-Hybrid System for Detecting Interacting Proteins

Abstract

Protein-protein interactions play an essential role in all living systems, and hence their analysis is of foremost importance in molecular biology. Although there are num-ber of methods to detect protein-protein interactions, the yeast two-hybrid system is probably the most successful method. Recently established protein interaction data- bases draw their data to a large extent from the summed input of small and large-scale two-hybrid screens.