The effect of cadmium on the activity of stress-related enzymes and the ultrastructure of pea roots

Abstract

The analysis of the effects of cadmium (Cd) on plant cells is crucial to understand defense mechanisms and adaptation strategies of plants against Cd toxicity. In this study, we examined stress-related enzyme activities after one and seven days of Cd application and the ultrastructure of roots of Pisum sativum L. after seven days of Cd treatment (10, 50, 100, and 200 µM CdSO4). Our results showed that phenylalanine ammonia-lyase (PAL) activity and the amount of Cd accumulated in the roots were significantly positively correlated with the Cd concentration used in our experiment. However, Cd caused a decrease of all studied antioxidative enzyme activities (i.e., catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX)). The analysis of the ultrastructure (TEM) showed various responses to Cd, depending on Cd concentrations. In general, lower Cd concentrations (50 and 100 µM CdSO4) mostly resulted in increased amounts of oil bodies, plastolysomes and the accumulation of starch granules in plastids. Meanwhile, roots treated with a higher concentration of Cd (200 µM CdSO4) additionally triggered protective responses such as an increased deposition of suberin lamellae in the endodermal cell walls. This indicates that Cd induces a complex defense response in root tissues.