Abstract
Chimeric antigen receptor T cell (CAR-T) therapies have now entered mainstream clinical practice with two approved autologous CAR-T products targeting CD19 and numerous other products in early and late phase clinical trials. This has led to a demand for highly sensitive, specific, and easily reproducible methods to monitor CAR-T cells in patients. Here we describe a flow cytometry based protocol for detection of allogeneic CAR-T cells and for monitoring their phenotype and numbers in blood and bone marrow of patients following CAR-T treatment.
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Jozwik, A., Dunlop, A., Sanchez, K., & Benjamin, R. (2020). Monitoring allogeneic CAR-T cells using flow cytometry. In Methods in Molecular Biology (Vol. 2097, pp. 293–308). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0203-4_19
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