Evaluation of Antioxidants, Antidiabetic, Antiinflammatory Active Compounds from Leptogium rivurale Through In-Vitro and In-Silico Studies

Abstract

Leptogium rivurale is a flooded jelly skin lichen as the surface becomes jelly on wet. It is a cyanolichen with a cyanobacterium Nostoc. In this present study, Leptoguium rivurale were collected from Kodaikanal and extracted using methanol and distilled water. Qualitative analysis of phytochemicals from the extracts showed the presence of carbohydrates, glycosides, phenols, terpenoids, saponins, and proteins. The methanol extract was found to inhibit the α-amylase enzyme activity better than aqueous extract. The methanol extract was found to have better DPPH radical scavenging activity and anti-inflammatory activity than aqueous extract. Then the extract was subjected to GC-MS analysis. The molecules obtained through GC-MS analysis were subjected to in-silico molecular docking simulation using AutoDock software. Cyclohexanol and oxirane were the potential drug candidates identified.