Mass spectrometry for proteomics-based investigation using the zebrafi sh vertebrate model system

Abstract

The zebrafi sh (Danio rerio) is frequently being used to investigate the genetics of human diseases as well as resulting pathologies. Ease of both forward and reverse genetic manipulation along with conservation of vertebrate organ systems and disease causing genes has made this system a popular model. Many techniques have been developed to manipulate the genome of zebrafi sh producing mutants in a vast array of genes. While genetic manipulation of zebrafi sh has progressed, proteomics have been under-utilized. This review highlights studies that have already been performed using proteomic techniques and as well as our initial proteomic work comparing changes to the proteome between the ascl1a −/− and WT intestine.