Murine B Cell Response to TLR7 Ligands Depends on an IFN-β Feedback Loop

  • Green N
  • Laws A
  • Kiefer K
  • et al.
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Abstract

Type I IFNs play an important, yet poorly characterized, role in systemic lupus erythematosus. To better understand the interplay between type I IFNs and the activation of autoreactive B cells, we evaluated the effect of type I IFN receptor (IFNAR) deficiency in murine B cell responses to common TLR ligands. In comparison to wild-type B cells, TLR7-stimulated IFNAR−/− B cells proliferated significantly less well and did not up-regulate costimulatory molecules. By contrast, IFNAR1−/− B cells did not produce cytokines, but did proliferate and up-regulate activation markers in response to other TLR ligands. These defects were not due to a difference in the distribution of B cell populations or a failure to produce a soluble factor other than a type I IFN. Instead, the compromised response pattern reflected the disruption of an IFN-β feedback loop and constitutively low expression of TLR7 in the IFNAR1−/− B cells. These results highlight subtle differences in the IFN dependence of TLR7 responses compared with other TLR-mediated B cell responses.

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APA

Green, N. M., Laws, A., Kiefer, K., Busconi, L., Kim, Y.-M., Brinkmann, M. M., … Marshak-Rothstein, A. (2009). Murine B Cell Response to TLR7 Ligands Depends on an IFN-β Feedback Loop. The Journal of Immunology, 183(3), 1569–1576. https://doi.org/10.4049/jimmunol.0803899

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