Chapter 4: Production Strategies and Challenges with IgG-Based Bispecific Ab Formats

Abstract

Bispecific antibodies are being developed against a range of diseases and are gaining presence in the pipeline of pharmaceutical companies. Rather than a single format, they consist of a variety of different forms reflecting different mindsets favoring a simplified production process or a more native-like molecule. In this chapter we review the most common bispecific Ab formats from a production point of view: we group them according to the simplicity of the process they require and discuss how the different engineering solutions may impact diverse aspects such as the specific yield, thermostability, analytical assays, or purification strategies.