Encapsulation of proteins with high/low pI in PLGA-particles for oral delivery

Abstract

Polylactic co-glycolic acid (PLGA) is a biocompatible and biodegradable polymer used for controlled drug delivery of small molecules or macromolecules including protein. Proteins highly requires an advanced drug delivery system because of its sensitivity towards many factors such as temperature, hygroscopicity, and biological system. Particularly for oral dosage form, protein must be encapsulated in extremely low pH resistant particle. Protein's intrinsic characterization determines effectivity of the encapsulation. The recent method of PLGA particle preparation was more suitable for proteins with low pI. PLGA showed higher effectivity to encapsulate protein with low pI such as BSA (pI = 4.8) than that of high pI for instance lysozyme (pI = 11). In the study, protein release and physico-chemical characterizations of BSA loaded PLGA-particle were performed.