We describe a DNA-barcoded antibody sensing technique for single cell protein analysis in which the barcodes are photocleaved and digitally detected without amplification steps (Ullal et al., Sci Transl Med 6:219, 2014). After photocleaving the unique ~70mer DNA barcodes we use a fluorescent hybridization technology for detection, similar to what is commonly done for nucleic acid readouts. This protocol offers a simple method for multiplexed protein detection using 100+ antibodies and can be performed on clinical samples as well as single cells.
CITATION STYLE
Ullal, A. V., & Weissleder, R. (2015). Photocleavable dna barcoding antibodies for multiplexed protein analysis in single cells. In Methods in Molecular Biology (Vol. 1346, pp. 47–54). Humana Press Inc. https://doi.org/10.1007/978-1-4939-2987-0_4
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