Lipid bilayer formation on an ion image sensor and measurement of time response of potential dependency on ion concentration

Abstract

We aimed to establish an ion channel measurement system using a charge transfer type ion image sensor on which an artificial lipid bilayer membrane was formed. A supported lipid bilayer (SLB) was formed on the K+-sensitive membrane composed of polyvinyl chloride on the ion image sensor. Fluid SLB fully covered the sensor surface and consequently reduced the output potential of the ion sensor, keeping the Nernst response of the ion sensor. We measured the time course after changing K+ concentrations before and after the formation of a SLB. The temporal response of the output potential was decelerated after the SLB formation, because ion diffusion to the sensor surface was shielded by the SLB. We demonstrated the ion channel reconstruction and inhibitor addition using gramicidin and Ca2+