Heterogeneity of biologic responses of melanoma-specific CTL.

  • Fonteneau J
  • Le Dréan E
  • Le Guiner S
  • et al.
39Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.

Abstract

To better understand how Ag density influences the various biologic responses of CTL, we analyzed lysis and, at the single cell level, cytokine production by a panel of melanoma-specific CTL clones. Titration experiments done with peptide-pulsed TAP-deficient T2 cells indicated that: 1) Ag density affects both the fraction of responding cells and the amount of cytokine secreted by each cell. 2) Different responses have a relative Ag requirement that may vary between clones. Lysis and secretion of IFN-gamma, and for most clones' secretion of TNF-alpha, required lower Ag densities, by one or two logs, than IL-2 and granulocyte-macrophage CSF secretion. 3) In a significant fraction of IFN-gamma-secreting cells, IL-2 production is not induced. 4) A large fraction of cloned cells is refractory to lymphokine gene activation for about 2 wk after previous stimulation. Together these data indicate that CTL biologic responses are controlled by variable Ag thresholds and by additional parameters affecting activation requirements of each cell. A similar heterogeneity of cytokine responses was observed to Ag endogenously presented by melanoma cells. As a consequence, most melanoma lines, including those with the highest Ag expression, could trigger only low CTL fractions to secrete IL-2 and, also for most clones, granulocyte-macrophage CSF. This may be an important component of the inefficiency of specific CTL in cancer patients.

Cite

CITATION STYLE

APA

Fonteneau, J. F., Le Dréan, E., Le Guiner, S., Gervois, N., Diez, E., & Jotereau, F. (1997). Heterogeneity of biologic responses of melanoma-specific CTL. The Journal of Immunology, 159(6), 2831–2839. https://doi.org/10.4049/jimmunol.159.6.2831

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free