Neutralization enzyme immunoassay for influenza virus

36Citations
Citations of this article
20Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

A neutralization enzyme immunoassay (N-EIA) was developed for the detection of antibody titer rises in sera of patients infected with influenza A (H3N2) virus. In this N-EIA, a selected strain of influenza A (H3N2) virus was added to monolayers of LLC-MK2 cells in microtiter plates. After 24 h, the replicated virus could be demonstrated with a virus-specific enzyme- labeled monoclonal antibody. Preincubation of the influenza virus with convalescent-phase sera of patients infected with influenza A (H3N2) virus resulted 1 day later in decreased absorbance values that could be used for calculation of neutralization titers. From use of paired serum samples from 10 patients with a history of flu-like symptoms, the results obtained with N- EIA correlated well (r = 0.83) with those of the standard hemagglutination inhibition test.

Cite

CITATION STYLE

APA

Benne, C. A., Harmsen, M., De Jong, J. C., & Kraaijeveld, C. A. (1994). Neutralization enzyme immunoassay for influenza virus. Journal of Clinical Microbiology, 32(4), 987–990. https://doi.org/10.1128/jcm.32.4.987-990.1994

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free