High count rates with total internal reflection fluorescence correlation spectroscopy

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Abstract

We achieved photon count rates per molecule as high as with commonly used confocal fluorescence correlation spectroscopy instruments using a new total internal reflection fluorescence correlation spectroscopy system based on an epi-illumination configuration.

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Hassler, K., Anhut, T., Rigler, R., Gösch, M., & Lasser, T. (2005). High count rates with total internal reflection fluorescence correlation spectroscopy. Biophysical Journal, 88(1). https://doi.org/10.1529/biophysj.104.053884

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