Beta-1,3-galactosyl-O-glycosyl- glycoprotein beta-1,6-NAcetylglucosaminyltransferase 1 (GCNT1) (C2GnT-L) and beta-1,3-galactosyl-oglycosyl- glycoprotein beta-1,6-NAcetylglucosaminyltransferase 3 (GCNT4) (C2GnT-T)

Abstract

The appearance of a branch Galβ1-3(GlcNAcβ1-6)GalNAc in O-glycans has been demonstrated in many important biological processes such as T cell activation, T cell development, leukemia, immunodeficiencies (Tsuboi and Fukuda 2001), and cancer metastasis (Tsuboi et al. 2012). This branch is formed by a glycosyltransferase, core2 β1-6GlcNAc transferase (designated C2GnT) that catalyzes the transfer of GlcNAc to Galβ1-3GalNAc in O-glycans, resulting in the conversion of Galβ1-3GalNAc, core1 to Galβ1-3(GlcNAcβ1-6)GalNAc, core2 (Fig. 34.1).