Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.
CITATION STYLE
Vuoristo, K. S., Mars, A. E., van Loon, S., Orsi, E., Eggink, G., Sanders, J. P. M., & Weusthuis, R. A. (2015). Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production. Frontiers in Microbiology, 6(AUG). https://doi.org/10.3389/fmicb.2015.00849
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