Protein fractionation by preparative electrophoresis.

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Abstract

Preparative electrophoresis is a protein fractionation approach useful for the enrichment of low-abundance gene products. Preparative electrophoresis is usually performed in the PrepCell apparatus. Proteins are separated according to their size in a cylindrical gel in the presence of an ionic detergent. The method is particularly efficient for the enrichment of low-molecular-mass gene products. Preparative electrophoresis can be followed by proteomic analysis, and the proteins eluted from the preparative gel can be separated by two-dimensional gel electrophoresis and identified by mass spectrometry.

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Fountoulakis, M., & Dimitraki, P. (2008). Protein fractionation by preparative electrophoresis. Methods in Molecular Biology (Clifton, N.J.). https://doi.org/10.1007/978-1-60327-064-9_24

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