Spatial separation of parental genomes in preimplantation mouse embryos

144Citations
Citations of this article
118Readers
Mendeley users who have this article in their library.
Get full text

Abstract

We have used two different experimental approaches to demonstrate topological separation of parental genomes in preimplantation mouse embryos: mouse eggs fertilized with 5-bromodeoxyuridine (BrdU)-labeled sperm followed by detection of BrdU in early diploid embryos, and differential heterochromatin staining in mouse interspecific hybrid embryos. Separation of chromatin according to parental origin was preserved up to the four-cell embryo stage and then gradually disappeared. In F1 hybrid animals, genome separation was also observed in a proportion of somatic cells. Separate nuclear compartments during preimplantation development, when extreme chromatin remodelling occurs, and possibly in some differentiated cell types, may be associated with epigenetic reprogramming.

Cite

CITATION STYLE

APA

Mayer, W., Smith, A., Fundele, R., & Haaf, T. (2000). Spatial separation of parental genomes in preimplantation mouse embryos. Journal of Cell Biology, 148(4), 629–634. https://doi.org/10.1083/jcb.148.4.629

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free