On the Hydrazinolysis of Proteins and Peptides: A Method for the Characterization of Carboxyl-terminal Amino Acids in Proteins

Abstract

(1) A brief method for the characterization of carboxyl-terminal amino acids in peptides and proteins was investigated.(2) Several new amino acid hydrazides were synthesized.(3) Soveral di- and tri-peptides were hydrazinolyzed and identified their carboxylterminal amino acid.(4) The method was applied to beef insulin and tyrocidin for the demonstration of its applicability.