Several methods have been developed to enrich DNA for subsets of the genome prior to next-generation sequencing. These front-end enrichment strategies provide powerful and cost-effective tools for researchers interested in collecting large-scale genomic sequence data. In this review, I provide an overview of both general and targeted reduced representation enrichment strategies that are commonly used in tandem with next-generation sequencing. I focus on several key issues that are likely to be important when deciding which enrichment strategy is most appropriate for a given experiment. Overall, these techniques can enable the collection of large-scale genomic data in diverse species, providing a powerful tool for the study of evolutionary biology.
CITATION STYLE
Good, J. M. (2011). Reduced Representation Methods for Subgenomic Enrichment and Next-Generation Sequencing. In Methods in Molecular Biology (Vol. 772, pp. 85–103). Humana Press Inc. https://doi.org/10.1007/978-1-61779-228-1_5
Mendeley helps you to discover research relevant for your work.