Chromosome architecture needs to be investigated in relation with the chemical function of DNA. The kinetics of gene expression, DNA replication, and repair are driven by the mechanisms by which a functional nuclear protein finds its substrate in the nucleus. Single-particle tracking (SPT) is a method to quantify fluorescent molecules dynamics from the tracks of the single molecules recorded by high-resolution microscopes. SPT offers direct observation of the movement and single-molecule resolution. Usually SPT is performed on membranes because of higher contrast. Here, we introduce a novel method to record the trajectories of weakly fluorescent molecules in the nucleus of living cells. I-SPT uses some specific detection and analysis tools to enable the computation of reliable statistics on nuclear particle movement.
CITATION STYLE
Récamier, V. (2016). Intra-nuclear single-particle tracking (I-SPT) to reveal the functional architecture of chromosomes. In Methods in Molecular Biology (Vol. 1431, pp. 265–274). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3631-1_18
Mendeley helps you to discover research relevant for your work.