Presented here are two engineered bacterial biosensors for detecting and quantifying silver and copper ions. The biosensors contain a silver/copper resistance operon and a Green Fluorescent Protein gene that is strictly regulated through silver activated promoter regions normally found on a silver resistance gene (sil operon). The two biosensors efficiently detected silver and copper concentrations of 40 µM–300 µM and 20 µM–600 µM respectively. A strong correlation (R 2 = 0.90 or above) between silver/copper and GFP signal makes it possible to quantify the ions using a linear regression. At room temperature incubation, the GFP signal of the biosensors in Ag + saturated after 13 h. However, a detectable GFP signal was seen in 4 h.
CITATION STYLE
Martinez, A. R., Heil, J. R., & Charles, T. C. (2019). An engineered GFP fluorescent bacterial biosensor for detecting and quantifying silver and copper ions. BioMetals, 32(2), 265–272. https://doi.org/10.1007/s10534-019-00179-3
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